Ars region in TL-DNA on octopine type Ti plasmids |
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Authors: | Yasuhiro Suzuki and Tetsuo Iino |
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Affiliation: | (1) Laboratory of Genetics, Department of Biology, Faculty of Science, University of Tokyo, Hongo, 113 Tokyo, Japan;(2) Present address: Tokyo College of Biotechnology, Kitakoujiya 1-3-14, Ohtaku, 113 Tokyo, Japan;(3) Present address: School of Human Sciences, Waseda University, Mikajima 2-579-15, Tokorozawa, 359 Saitama, Japan |
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Abstract: | Summary In the TL-DNA region of the octopine type Ti plasmids, an ars region was assigned as the DNA segment conferring the replicational ability to YIp5 in Saccharomyces cerevisiae. T-DNA:YIp5 hybrid plasmids containing a particular T-DNA region could transform yeast cells at a frequency of 103–104 transformants per g plasmid DNA and they were rescued in Escherichia coli, although the transformed phenotype was mitotically unstable. The instability was inferred to be caused by segregation of the plasmids due to their low efficiency of replication. The ars region was mapped on the noncoding region between the coding regions corresponding to no. 5 and no. 7 mRNA, and its minimal length determined in this experiment was about 150 bp.Abbreviations Ti plasmid tumor inducing plasmid - T-DNA transferred DNA or tumor DNA - TL-DNA left T-DNA - ars autonomously replicating sequences |
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Keywords: | Agrobacterium tumefaciens Ti plasmid T-DNA Autonomously replicating sequences ars |
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