辣椒素引起脑干内心血管活动相关核团中c-fos的表达

在１６只切断两侧缓冲神经的大鼠,观察颈总动脉注射辣椒素对脑干内心血管活动相关核团ｃ－ｆｏｓ原癌基因表达的影响。在剂对照组大鼠脑干,仅见少数Ｆｏｓ蛋白样免疫反应（ＦＬＩ）神经元。与对照组相比,颈总动脉注射辣椒素（１０μｍｏｌ,０．１ｍｌ）时,脑干内巨细胞旁外侧核（ＰＧＬ）、蓝斑（ＬＣ）、最后区（ＡＰ）和孤束核（ＮＴＳ）等部位的ＦＬＩ神经元显著增加,而中脑中央灰质（ＰＡＧ）和中缝核群（ＲＮ）的ＦＬＩ神

Increased expression of c-fos in the brainstem nuclei involved in cardiova scular regulation by capsaicin

Effects of intracarotid injected capsaicin on the expression of Fos proto-oncogene in the brainstem nuclei involved in cardiovascular regulation were examined in 16 anesthetized rats with sinoaortic denervation. Only a few Fos-like protein immunoreactivity (FLI) neurons were found in the vehicle-control rats. Following intracarotid injection of capsaicin (10 micromol, 0.1 ml), the FLI neurons were markedly increased in nucleus paragigantocellularis lateralis (PGL), locus coeruleus (LC), area postrema (AP) and nucleus tractus solitarius (NTS), as compared with the results of vehicle-control rats, while the FLI neurons of raphe nuclei (RN) and periaqueductal gray (PAG) were not affected. The excitatory response to intracarotid injection of capsaicin was significantly inhibited by pretreatment with the vanilloid receptor (capsaicin receptor) antagonist ruthenium red (200 mmol, 0.1 ml). The results indicate that capsaicin may activate the neurons of the brainstem nuclei involved in cardiovascular regulation through vanilloid receptors, but does not exert any effects on PAG and RN.