Production of L-Malic Acid by Permeabilized Cells of Commercial Saccharomyces Sp. Strains |
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Authors: | Ana Vrsalović Presečki Đurđa Vasić-Rački |
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Institution: | Faculty of Chemical Engineering and Technology, University of Zagreb, Savska c. 16, HR-10000 Zagreb, Croatia. |
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Abstract: | Of various yeasts tested in the conversion of fumaric to L-malic acid, Saccharomyces bayanus had the highest activity of fumarase. Cells permeabilized with 0.2% (w/v) CTAB for 5 min gave maximum enzyme activity. Under non-growth conditions, fumarase activity in the permeabilized cells was four times higher (271 U/g) than that of the intact cells (67 U/g). The proposed mathematical model for the batch production of L-malic acid was validated at different initial fumaric acid concentrations. The average conversion of fumaric acid was up to 82% and gave 21, 40, 83 and 175 mM L-malic acid from respectively, 25, 50, 100 and 210 mM: fumaric acid. |
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