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Cloning and activation of the Syrian hamster neu proto-oncogene |
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| Authors: | Takuro Nakamura Toshikazu Ushijima Yukihito Ishizaka Minako Nagao Masami Aral Yukari Yamazaki and Takatoshi Ishikawa |
| Institution: | a Department of Pathology, Faculty of Medicine, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan. Tel. (81-3) 3812-2111 b Carcinogenesis Division, National Cancer Center Research Institute, Tsukiji. Chuo-ku, Tokyo 105., Japan. Tel. (81-3) 3542-2511 |
| Abstract: | Point mutations of the Syrian hamster neu proto-oncogene have been observed in the transmembrane domain of N-nitroso-N-ethylurea(ENU)-induced neurofibromas, Genomic DNA derived from tumor tissue showed transforming activity in an NIH 3T3 assay and a cDNA clone of the hamster neu gene, containing the entire protein-coding region, was isolated from a transformant cDNA library. The encoded product is 92 and 88% homologous to the rat neu and the human c-erbB-2, respectively. The product of the mutated hamster neu gene showed increased autophosphorylation of Tyr residues. |
| Keywords: | Receptor tyrosine kinase complementary DNA Schwarm cell neurofibroma transforming activity tyrosine phosphorylation |
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