Raffinose and hypotaurine improve the post-thawed Merino ram sperm parameters |
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| Authors: | Mustafa Numan Bucak,Nazan Keskin,Mehmet Taşpınar,Kenan Ç oyan,Nuri Başpınar,Mihai C. Cenariu,Ali Bilgili,Caner Ö ztü rk,Ahmet Nuri Kurşunlu |
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| Affiliation: | 1. Selcuk University, Veterinary Faculty, Department of Reproduction and Artificial Insemination, Konya, Turkey;2. Pamukkale University, Medical Faculty, Department of Histology and Embriyology, Denizli, Turkey;3. Yuzuncu Y?l University, Medical Faculty, Department of Medical Biology, Van, Turkey;4. Selcuk University, Veterinary Faculty, Department of Biochemistry, Konya, Turkey;5. Agricultural Sciences and Veterinary Medicine University, Veterinary Medicine Faculty, Department of Animal Reproduction, Cluj-Napoca, Romania;6. Ankara University, Veterinary Faculty, Department of Pharmacology and Toxicology, Ankara, Turkey;g Selcuk University, Science Faculty, Department of Chemistry, Konya, Turkey |
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| Abstract: | The aim of this study was to determine the effects of raffinose and hypotaurine on sperm parameters after the freeze–thawing of Merino ram sperm. Totally 40 ejaculates of five Merino ram were used in the study. Semen samples, which were diluted with a Tris-based extender containing 10 mM raffinose, 5 mM hypotaurine, 5 mM raffinose +2.5 mM hypotaurine (H + R) and no antioxidant (control), were cooled to 5 °C and frozen in 0.25 ml French straws and stored in liquid nitrogen. Frozen straws were then thawed individually at 37 °C for 25 s in a water bath for evaluation. |
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| Keywords: | Ram sperm Additives Motility Flourescent staining parameters Comet test TUNEL-positive cells |
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