The effect of cysteine and superoxide dismutase on the quality of post-thawed chicken sperm |
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| Authors: | Agnieszka Partyka Wojciech Niżański Joanna Bajzert Ewa Łukaszewicz Małgorzata Ochota |
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| Institution: | 1. Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Department of Reproduction and Clinic of Farm Animals, pl. Grunwaldzki 49, 50-366 Wroc?aw, Poland;2. Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Department of Immunology, Pathophysiology and Prevention Veterinary, ul. C.K. Norwida 31, 50-375 Wroc?aw, Poland;3. Wroclaw University of Environmental and Life Sciences, Faculty of Biology and Animal Breeding, Department of Poultry Breeding, ul. Che?mońskiego 38c, 51-630 Wroc?aw, Poland |
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| Abstract: | The study was conducted to determine the influence of N-acetyl-l-cysteine (NAC) and superoxide dismutase (SOD) on chicken sperm motility, plasma membrane and acrosome integrity, mitochondrial activity, lipid peroxidation (LPO) and apoptotic changes after freezing–thawing process. Semen samples from fifteen Greenlegged Partridge roosters were pooled, diluted with EK extender without antioxidants (control), or supplemented with 5 mM NAC, or 200 U/mL SOD. Samples were subjected to cryopreservation. After thawing, sperm parameters evaluated by using CASA system and flow cytometry were assessed. |
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| Keywords: | Antioxidants Avian semen Cryopreservation Cysteine Superoxide dismutase |
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