Molecular cloning, sequence analysis and expression studies of a novel GAmyb homologous gene, hvmyb |
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Authors: | LING Shiyun QI Fang ZHU Yuxian |
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Affiliation: | (1) State Key Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, 100871 Beijing, China |
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Abstract: | Using a knownGAmyb gene as the probe, two fully identical clones were isolated from a barley aleurone cDNA library. Sequence analysis showed that their 5′ termini are highly homoIogous to the 3′ termini ofGAmyb (97%) and their 3′ termini share no significant homology with any myb genes. Therefore, the deduced protein may hold intact putativeGAmyb activation domain but lack the normal DNA-binding domain. Northern blot reveals thathumyb expression in barley aleurone layers is strongly up-regulated by gibberellin (GA) and down-regulated by abscisic acid (APIA). The tissue-and developmental-stage-specificity ofhvmyb was also found, which was only expressed in barley aleurone cells and dropped to non-detectable level soon after germination. EMBL accession number Y14658. |
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Keywords: | barley aleurone GAmyb GA ABA |
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