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A mariner-based transposition system for Listeria monocytogenes
Authors:Cao Min  Bitar Alan Pavinski  Marquis Hélène
Affiliation:Department of Microbiology and Immunology, VMC C5-169, Cornell University, Ithaca, NY 14853-6401, USA.
Abstract:In this study, we developed a new mariner-based transposition system for Listeria monocytogenes. The mariner-based system has a high rate of transposition and a low rate of plasmid retention, and transposition is very random, making it an ideal tool for high-throughput transposon mutagenesis in L. monocytogenes.
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