A mariner-based transposition system for Listeria monocytogenes |
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Authors: | Cao Min Bitar Alan Pavinski Marquis Hélène |
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Affiliation: | Department of Microbiology and Immunology, VMC C5-169, Cornell University, Ithaca, NY 14853-6401, USA. |
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Abstract: | In this study, we developed a new mariner-based transposition system for Listeria monocytogenes. The mariner-based system has a high rate of transposition and a low rate of plasmid retention, and transposition is very random, making it an ideal tool for high-throughput transposon mutagenesis in L. monocytogenes. |
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