HindIII and Sst I restriction sites mapped on rabbit poxvirus and vaccinia virus DNA. |
| |
| Authors: | R Wittek, A Menna, D Schü mperli, S Stoffel, H K Mü ller, R Wyler |
| |
| Affiliation: | R Wittek, A Menna, D Schümperli, S Stoffel, H K Müller, and R Wyler |
| |
| Abstract: | The DNAs of two closely related orthopoxviruses, rabbit poxvirus (RPV) and vaccinia virus (VV), were mapped by overlapping-fragment analysis using restriction endonucleases HindIII and Sst I. The exact arrangement of these fragments was accomplished by total digestion of isolated partial restriction products and by end-fragment determination. RPV and VV DNAs showed identical restriction patterns in an internal region comprising approximately 60% of the genome. The size, by electrophoretical analysis of the RPV DNA, was 118 X 10(6) daltons, some 6 X 10(6) daltons less than VV DNA. The two opposite terminal restriction fragments of RPV DNA cross-hybridized to each other. |
| |
| Keywords: | |
|
|
