The sheep antibody response to repeated infection with Lucilia cuprina. |
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Authors: | D. S. Seaton T. J. O'meara R. A. Chandler R. M. Sandeman |
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Affiliation: | School of Agriculture, La Trobe University, Bundoora, Victoria 3083, Australia |
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Abstract: | , , and 1992. The sheep antibody response to repeated infection with Lucilia cuprina. International Journal for Parasitology 22: 1169–1174. The specific serum antibody responses of sheep exposed to 10 consecutive infections of L. cuprina have been analysed by enzyme-linked immuno-sorbent assay and immunoblotting using monoclonal antibodies specific for sheep immunoglobulin isotypes. Recognition of a number of larval excretory-secretory products by IgM antibodies appeared to be non-specific. IgG1 was the major antibody class stimulated by the infection protocol and marked increases in antibody to specific excretory-secretory antigens were observed. Three molecules of 35, 30 and 25 kDa were particularly recognized although the extent of recognition of these molecules varied considerably between individual sheep serum. A pooled serum composed of sera collected after five to seven infections significantly inhibited larval growth in in vitro cultures when compared to a sera pool consisting of sera collected both prior to infection and after infections 1 and 2. The degree of inhibition was greater when serum with high specific antibody titre was used. |
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Keywords: | Lucilia cuprina sheep antibody specificity ELISA immunoblotting in vitro culture |
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