| 培养原始生殖细胞的新方法 |
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| 引用本文： | 路欣 许晴 史小林. 培养原始生殖细胞的新方法[J]. 实验生物学报, 2005, 38(6): 474-480 |
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| 作者姓名： | 路欣 许晴 史小林 |
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| 作者单位： | 首都医科大学生殖医学研究中心,北京100069 |
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| 基金项目： | 基金项目北京市教委科技发展计划资助课题（KM200310025089）. |
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| 摘 要： | 为探讨原始生殖细胞（primordial germ cells，PGCs）在体外长期增殖、生长并长期保持分化潜能的新方法，我们将PGCs分别与睾丸支持细胞（Sertoli cells，SCs）和同源生殖嵴成纤维细胞共培养。结果与SCs共培养的PGCs集落明显多于同源生殖嵴成纤维细胞共培养PGCs集落，传代次数也显著多于同源生殖嵴成纤维细胞，目前与SCs共培养的PGCs已成功传代培养至了第51代。因此我们认为PGCs与SCs共培养．可有效提高原始生殖细胞在体外的增殖能力并可长期维持干细胞的特性。
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| 关 键 词： | 睾丸支持细胞 细胞培养 原始生殖细胞 |
| 收稿时间： | 2004-12-14 |
| 修稿时间： | 2004-12-142005-08-30 |
A NEW CULTURE METHOD FOR PRIMORDIAL GERM CELL |
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| LU Xin ,XU Qing ,SHI Xiao Lin. A NEW CULTURE METHOD FOR PRIMORDIAL GERM CELL[J]. Acta Biologiae Experimentalis Sinica, 2005, 38(6): 474-480 |
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| Authors: | LU Xin XU Qing SHI Xiao Lin |
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| Affiliation: | Reproductive Medical Center,Capital University of Medical Sciences, Beijing 100069 |
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| Abstract: | To investigate the new method that the primordial germ cells (PGCs) can long time propagate, grow and keep potency of differentiation in vitro. PGCs were co-cultured with Sertoli cells (SCs) or with homologue fibroblasts from genital ridge. When co-cultured with SCs, PGCs colonies and times of transfer of culture were greater than those co-cultured with homologue fibroblasts. At present PGCs of co-cultured with SCs have succeeded in subculturing to the 51 generation. SCs can availably enhance propagating ability as well as a long period of time maintain the characteristic of PGCs. |
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| Keywords: | Sertoli cells. Cell culture. Primordial germ cells |
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