Morphology and Small Subunit rDNA Gene Sequence of Pseudoamphisiella quadrinucleata n. sp. (Ciliophora,Urostylida) from the South China Sea |
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| Authors: | ZHUO SHEN XIAOFENG LIN HONGAN LONG MIAO MIAO HONGBIN LIU KHALED A S AL‐RASHEID WEIBO SONG |
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| Institution: | 1. Laboratory of Protozoology, College of Life Science, South China Normal University, Guangzhou 510631, China, and;2. Department of Biology, The Hong Kong University of Science and Technology, Kowloon, Hong Kong, and;3. Laboratory of Protozoology, Ocean University of China, Qingdao 266003, China, and;4. Zoology Department, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia |
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| Abstract: | ABSTRACT. The urosylid genus Pseudoamphisiella was established by Song (1996) with hitherto only two known congeners. In the present work, the morphology and infraciliature of a new member, Pseudoamphisiella quadrinucleata n. sp., a form with conspicuous alveolar layer and four macronuclear nodules isolated from the coastal waters both near Hong Kong and near Guangzhou, South China were investigated using living observation and protargol silver impregnation methods. Pseudoamphisiella quadrinucleata differs from other two known forms mainly by the number of macronuclear nodules: constantly four vs. two in Pseudoamphisiella alveolata and 24–57 in Pseudoamphisiella lacazei. To support this, the sequence of the small subunit rDNA of P. quadrinucleata n. sp. showed 14 and 74 nucleotides in comparison with that of the two known congeners, respectively, which hence firmly supports the validity of the new species. |
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| Keywords: | Hypotrich marine ciliate new species Pseudoamphisiellidae taxonomy |
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