Smoking has no effect on the amino acid composition of apolipoprotein B100 of LDL while directly influencing the antioxidant status |
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Authors: | Sekher Pannala Ananth Bruckdorfer K Richard Rice-Evans Catherine A |
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Affiliation: | Wolfson Centre for Age Related Diseases, GKT School of Biomedical Sciences, King's College London, St. Thomas' Street, London SE1 9RT, United Kingdom. |
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Abstract: | Previous studies have demonstrated increased plasma levels of oxidised low-density lipoprotein (oxLDL) in chronic smokers, which has been associated with the extent of endothelial dysfunction. In this study we examine the relationship between the amino acid composition of apolipoprotein B100 (apo B) of low-density lipoprotein (LDL), by reverse phase HPLC after precolumn derivatisation, between smokers (> or =40 cigarettes/day) and nonsmokers in relation to their plasma and LDL antioxidant status. While there was a significant difference in the levels of plasma vitamin C and alpha-tocopherol between female smokers and nonsmokers, as well as in the levels of LDL alpha-tocopherol, there was no significant difference in the amino acid composition of apo B between the two groups. |
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Keywords: | smoking vitamin C α-tocopherol amino acid analysis protein hydrolysis precolumn derivatisation o-phthalaldehyde apolipoprotein B100 |
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