Microarray analysis identifies genes preferentially expressed in the lung schistosomulum of Schistosoma mansoni |
| |
Authors: | Dillon Gary P Feltwell Theresa Skelton Jason P Ashton Peter D Coulson Patricia S Quail Michael A Nikolaidou-Katsaridou Nefeli Wilson R Alan Ivens Alasdair C |
| |
Institution: | Department of Biology, University of York, PO Box 373, York YO10 5YW, UK. |
| |
Abstract: | The lung schistosomulum of Schistosoma mansoni is a validated target of protective immunity elicited in vaccinated mice. To identify genes expressed at this stage we constructed a microarray, representing 3088 contigs and singlets, with cDNA derived from in vitro cultured larvae and used it to screen RNA from seven life-cycle stages. Clustering of genes by expression profile across the life cycle revealed a number of membrane, membrane-associated and secreted proteins up-regulated at the lung stage, that may represent potential immune targets. Two promising secreted molecules have homology to antigens with vaccine and/or immunomodulatory potential in other helminths. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|