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Human granulocyte specific nuclear antigen(s). I: Production of antisera and determination of specificity.
Authors:R C Briggs  J F Chiu  L S Hnilica  F Chytil  L W Rogers  D L Page
Affiliation:1. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Rd., Wuhan, 430079, China;2. Department of Orthodontics, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Rd., Wuhan, 430079, China;3. Hospital and School of Stomatoloy, Xiamen Medical University, Xiamen, 361003, China;4. School of Dentistry, The University of Queensland, Brisbane, QLD, 4006, Australia;1. Department of Chemistry, College of Science, King Faisal University, Al-Ahsa, 31982, Saudi Arabia;2. Chemistry Department, Faculty of Science, Sohag University, Sohag, 82524, Egypt;1. Dipartimento di Scienze Farmaceutiche, Università degli Studi di Milano, Milano, Italy;2. DeFENS - Dipartimento di Scienze per gli Alimenti, la Nutrizione e l’Ambiente, Università degli Studi di Milano, Milano, Italy
Abstract:Non-histone protein-DNA complexes isolated from human lung tissue were used to immunize New Zealand rabbits. Quantitative microcomplement fixation and immunocytochemistry revealed a specific chromatin antigen(s) in the human granulocyte nucleus. The study demonstrates the feasibility of employing antisera against chromosomal non-histone protein-DNA complexes for the immunochemical identification of cell types.
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