Surprising duplex stabilisation upon mismatch introduction within triply modified duplexes |
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Authors: | Mieke A. Catry |
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Affiliation: | Ghent University, Department of Organic Chemistry, Krijgslaan 281 S4, 9000 Gent, Belgium |
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Abstract: | Three different modified phosphoramidite nucleoside building blocks equipped with additional protected imidazole, masked alcohol and masked carboxylate functionality are synthesized and incorporated into oligonucleotides. Based on the serine-protease active site model, doubly and triply modified duplexes are created and tested for stability. Analysis of different spatial distributions of the extra functionalities shows that careful positioning can even overcome duplex destabilisation caused by the introduction of mismatches. |
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Keywords: | Serine proteases Modified oligonucleotides Enzyme models |
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