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Development and characterization of polymorphic microsatellite DNA markers for Sasa senanensis (Poaceae: Bambuseae) |
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| Authors: | Yuko Miyazaki Naoki Ohnishi Kimiko Hirayama Junco Nagata |
| Affiliation: | (1) Nara Forest Research Institute, Takatori 635-0133, Japan;(2) Kansai Research Center, Forestry and Forest Products Research Institute, Nagaikyutaro 68, Kyoto 612-0855, Japan;(3) Present address: Department of Life and Environmental Sciences, Kyoto Prefectural University, 1-5, Hangi-cho, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan;(4) Forestry and Forest Products Research Institute, Tsukuba 305-8687, Japan |
| Abstract: | Sasa senanensis is a dwarf bamboo species distributed on the floors of cool temperate forests in Japan and adjacent regions. We isolated eight polymorphic microsatellite loci from this species. The number of alleles ranged from two to eight and the observed heterozygosity per locus from 0.13 to 0.74. Seven of the eight loci were also polymorphic in Sasa nipponica. Most of these markers were successfully amplified in other dwarf bamboo species. These markers will be useful for investigating clonal structure and population genetics in some dwarf bamboo species. |
| Keywords: | Clonal plant Dwarf bamboo Cross-species amplification Sasa nipponica |
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