Chemically modified oligonucleotides with efficient RNase H response |
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Authors: | Vester Birte Boel Anne Marie Lobedanz Sune Babu B Ravindra Raunkjaer Michael Lindegaard Dorthe Raunak Hrdlicka Patrick J Højland Torben Sharma Pawan K Kumar Surender Nielsen Poul Wengel Jesper |
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Affiliation: | Nucleic Acid Center, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark. |
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Abstract: | Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3-ON12 and ON15-ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage. |
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