Amplification of the translocated c-myc genes in three Burkitt lymphoma cell lines |
| |
| Institution: | 1. Department of Medical Genetics, University of Alberta, Edmonton, AB, Canada;2. Department of Pathology, University Health Network, Toronto, Canada;3. Department of Pathology and Molecular Medicine, Queen''s University, Kingston, Canada;4. Departments of Genetics, School of Medicine of Ribeirão Preto, University of Sao Paulo, Ribeirão Preto, São Paulo, Brazil;5. Department of Physics, Engineering Physics and Astronomy, Queen''s University, Kingston, Canada;6. Genetics Diagnostic Laboratory, Children''s Hospital of Eastern Ontario, Ottawa, Canada;7. Department of Pathology and Laboratory Medicine, University of Ottawa, Ottawa, Canada;8. Department of Pathology and Legal Medicine, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil;1. LBME, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, 31062 Toulouse, France;2. LBCMCP, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, 31062 Toulouse, France;1. INSERM U932, Institut Curie, ANR-10-IDEX-0001-02 PSL* and ANR-11-LABX-0043, Paris, France;2. Institut Curie, PSL Research University, CNRS, UMR 144, F-75005 Paris, France;3. Institut Pierre-Gilles de Gennes, PSL Research University, F-75005 Paris, France;4. Laboratoire Jean Perrin, UM 8237 CNRS/UPMC, 4 place Jussieu, 75005 Paris, France;1. Departments of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94143, USA;2. Division of Pediatric Hematology and Oncology, Department of Pediatrics, Oregon Health & Science University, Portland, OR 97239, USA;3. Knight Cancer Institute, Oregon Health & Science University, Portland, OR 97239, USA;4. Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA;5. Papé Family Pediatric Research Institute, Oregon Health & Science University, Portland, OR 97239, USA;6. Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, NY 10065, USA;7. Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei 11221, Taiwan;8. MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, OX3 9DS, UK;9. Departments of Medicine and Pharmacology, Weill Cornell Medical College, New York, NY 10065, USA;10. Department of Leukemia, University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA;11. Hôpital Maisonneuve-Rosemont, Montreal, QC H1T 2M4, Canada;12. Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA;13. Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA;14. Division of Pediatric Oncology and Center for Childhood Cancer Research, Children’s Hospital of Philadelphia, Philadelphia, PA 19104, USA;15. Department of Cell & Developmental Biology, Oregon Health & Science University, Portland, OR 97239, USA;16. Pediatric Hematology-Oncology, University of California, San Francisco, San Francisco, CA 94143, USA;17. Howard Hughes Medical Institute, Portland, OR 97239, USA;1. Department of Urology, Affiliated Hospital of Kunming University of Science and Technology, The First People’s Hospital of Yunnan Province, PR China;2. Faculty of Life Science and Technology, Kunming University of Science and Technology, PR China;1. Department of Orthodontics, Medical Faculty, University of Bonn, 53111 Bonn, Germany;2. Department of Oral & Maxillofacial Plastic Surgery, University of Bonn, 53105 Bonn, Germany;3. Institute of Reconstructive Neurobiology, Life and Brain Center, University of Bonn, 53127 Bonn, Germany |
| |
| Abstract: | Translocations of the coding exons of the human c-myc gene are consistent features of human Burkitt lymphomas (BL). In the BL cell lines CA46, JD40, and ST486, the second and third c-myc exons have been translocated into the immunoglobulin heavy chain locus. In addition to this rearrangement, in all three cell lines, we have found that the translocated c-myc exons show low-level amplification relative to restriction fragments from the germ-line c-myc gene. The patterns of hybridization of an IgM switch region probe suggest that immunoglobulin heavy chain sequences have been co-amplified with the translocated c-myc sequences. Differential sedimentation was used to determine whether the amplified sequences reside in high-molecular-weight chromosomes or low-molecular-weight extrachromosomal DNA. In JD40 and ST486 cells, the amplified c-myc sequences were found on high-molecular-weight chromosomes; ST486 cells also contained translocated c-myc sequences in low-molecular-weight, extrachromosomal DNA, as did CA46 cells. These conclusions were corroborated by fluorescence in-situ hybridization (FISH) of HeLa, CA46, ST486 and JD40 metaphase chromosomes. These results suggest that there is ongoing selection for cells containing amplified copies of the expressed c-myc sequences, and that there is continuous generation of extrachromosomal copies of the translocated c-myc sequences in ST486 and CA46 cells. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
