Determination of quinolone antibiotics in growth media by reversed-phase high-performance liquid chromatography |
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| Affiliation: | 1. University of Minnesota, College of Pharmacy, Minneapolis MN, and St. Paul-Ramsey Medical Center, Clinical Pharmacy, 640 Jackson Street, St. Paul, MN 55101, USA;2. Medtox Laboratories, Inc., New Brighton, MN, USA;3. Scientific Research Consortium, Inc., St. Paul, MN, USA;1. College of Chemistry and Material Science, Fujian Normal University, Fuzhou 350007, PR China;2. Fujian Key Laboratory of Polymer Materials, Fuzhou 350007, PR China;1. Beijing National Laboratory of Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China;2. Graduate School, University of Chinese Academy of Sciences, Beijing 100049, PR China;3. Beijing National Laboratory of Molecular Sciences, Lab of Polymer Physics and Chemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China;4. Graduate School, Shandong Agricultural University, 271018 Shandong, PR China;1. College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China;2. School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou 510006, China |
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| Abstract: | A simple, accurate, precise, and versatile high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of three quinolone antibiotics in Mueller–Hinton broth. The fluoroquinolone agents studied were ciprofloxacin, ofloxacin, and sparfloxacin; other quinolone agents have been identified using this method but not validated in this matrix (levofloxacin, clinafloxacin, temafloxacin, and trovafloxacin). In addition, several other biological growth mediums have been investigated (human serum, human urine, Todd–Hewitt growth media, Ensure enteral feeding solution, and Haemophilus growth media). This method uses UV detection (280 nm), a simple, one-step protein precipitation extraction, and separation using a C18 column with an isocratic, ion-pairing mobile phase. An appropriate internal standard was obtained by using another quinolone antibiotic of differing retention time. The calibration curves were linear (r2≥0.999) over a concentration range of 0.0625–20.0 μg/ml with a lower limit of quantification of 0.1 μg/ml. The intra-day and inter-day coefficients of variation were less than 15%. |
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