A physical map of the genome of ethanol fermentative bacterium Zymomonas mobilis ZM4 and localization of genes on the map |
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| Institution: | 1. QGASLAB, Department of Mathematics, University of Cape Town, Rondebosch 7701, Cape Town, South Africa;2. CAMGSD, Departamento de Matemática, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal;3. Institute of Physics, Jagiellonian University, Ul. ?ojasiewicza 11, 30-348 Kraków, Poland;1. Central Siberian Botanical Garden, Siberian Branch, Russian Academy of Sciences, Novosibirsk;2. Voevodsky Institute of Chemical Kinetics and Combustion, Siberian Branch, Russian Academy of Sciences, Novosibirsk;3. Budker Institute of Nuclear Physics, Siberian Branch, Russian Academy of Sciences, Novosibirsk;1. National Consultant Laboratory for Hantaviruses, Institute of Medical Virology, Helmut-Ruska-Haus, Charité—University Medicine, Charitéplatz 1, 10117 Berlin, Germany;2. GBD Gesellschaft für Biotechnologische Diagnostik mbH, Potsdamer Chaussee 80, 14129 Berlin, Germany;3. INSTAND e.V., Society for Promoting Quality Assurance in Medical Laboratories, Ubierstrasse 20, 40223 Düsseldorf, Germany;4. Institute of Virology, Campus Benjamin Franklin, Charité—University Medicine, Hindenburgdamm 27, 12203 Berlin, Germany;1. Connected and Mobile Systems, Institute of Operating Systems and Computer Networks, TU Braunschweig, Mühlenpfordtstr. 23, Braunschweig, Germany;2. Ambient Intelligence, Department of Communications and Networking, Aalto University, Maarintie 8 Espoo, Finland;1. Division of Plastic and Hand Surgery, University Hospital Zurich, Switzerland;2. Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zurich, CH-8093 Zurich, Switzerland;3. Division of Trauma Surgery, University Hospital Zurich, Switzerland;4. Division of Thoracic Surgery, University Hospital Zurich, Switzerland;1. Centre de Biologie et de Pathologie Est, Département de Biochimie et Biologie Moléculaire, GHE, Hospices Civils de Lyon, F-69677, Bron Cedex, France;2. Univ-Lyon, CarMeN Laboratory, Inserm U1060, INRA U1397, Université Claude Bernard Lyon 1, INSA Lyon, F-69100, Villeurbanne, France;3. Centre de Biologie Sud, Laboratoire de Biochimie Moléculaire et Métabolique, Hospices Civils de Lyon, Centre Hospitalier Lyon-Sud, F-69310, Pierre-Bénite, France;4. Fédération d''endocrinologie, Maladies Métaboliques, Diabète et Nutrition, Hôpital Louis Pradel, Hospices Civils de Lyon, F-69677, Bron Cedex, France;5. CENS, Centre de Recherche en Nutrition Humaine Rhône-Alpes, F-69921, Oullins Cedex, France |
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| Abstract: | A physical map of the Zymomonas mobilis ZM4 genome has been constructed from the results of reciprocal Southern hybridization with PmeI, PacI, and NotI-digested genomic DNA fragments and linking cosmid clones. Restriction enzyme-digested Z. mobilis ZM4 genome was electrophoresed with phage lambda DNA concatemers as a size standard in a Bio-Rad CHEF-DRII pulsed-field gel electrophoresis (PFGE) system. The restriction enzyme PmeI generated 15 fragments (3–625 kb), and PacI produced 19 fragments (7–525 kb). Each size of restriction fragment was calculated by comparison to the size of phage lambda DNA concatemers, and the genome size of Z. mobilis ZM4 was estimated to be 2085.5 kb. The 19 known genes and three rrn operons were localized on the map. |
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