Pea polyubiquitin genes: (I) structure and genomic organization |
| |
| Institution: | 1. Department of Agronomy, Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China;2. China National Rice Research Institute (CNRRI), Fuyang 311400, PR China;1. State Key Laboratory of Molecular Engineering of Polymers and Department of Macromolecular Science, Fudan University, Shanghai 200433, China;2. Collaborative Innovation Center of Chemistry for Energy Materials, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Department of Chemistry, Fudan University, Shanghai 200433, China;3. School of Materials Science and Energy Engineering, Foshan University, Jiangwan First Road, Foshan 528000, Guangdong, China;4. BYD Company Limited, Kengzi Sub-district, Shenzhen 518133, Guangdong, China;1. School of Chemical Engineering, Anhui University of Science and Technology, 168 Taifeng Street, Huainan, Anhui 232001, China;2. State Key Laboratory of Mining Response and Disaster Prevention and Control in Deep Coal Mines, AUST, 168 Taifeng St., Huainan, Anhui 232001, China;3. Department of Safety, Health and Environmental Engineering, Hung-Kuang University, Taichung 43302, Taiwan |
| |
| Abstract: | Four polyubiquitin genes, PUB1, PUB2, PUB3 and PUB4, were isolated from a pea (Pisum sativum L. cv Alaska) genomic library and completely sequenced. They represent all of the four polyubiquitin genes of the ubiquitin gene family in pea. The coding regions of the genes contain five or six coding units arranged as tandem repeats. The different coding repeats of the four genes share homologies between 75 and 97%, encoding the same protein of 76 amino acids identical to those from other higher plants. The open reading frames of PUB1, PUB2 and PUB4 terminate in the additional amino acid, phenylalanine (F), and PUB3 terminates in isoleucine (I). The polyubiquitin genes all contain intron sequences ranging from 584 to 1114 bp immediately 5′ to the ATG initiation codon of the first coding sequence. Of the four genes, three are associated with long AT-rich (85.4–89.4% A+T) sequences ranging from about 331 to 478 bp at their 5′ or 3′ ends. The PUB4 gene was found to be linked to a moderate to highly repetitive DNA at its 5′ flanking sequence. The greater sequence homology between different genes than among individual repeating units of a gene suggests that the polyubiquitin genes may have arisen by gene duplication of a single gene sequence. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
