Identification and characterization of kraken,a gene encoding a putative hydrolytic enzyme in Drosophila melanogaster |
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| Affiliation: | 1. Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK;2. Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK;1. Division of Biology and Bioengineering, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125, USA;1. Department of Clinical Laboratory, Children''s Hospital of Shanghai, Shanghai Jiaotong University, Shanghai, China;2. Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China;1. College of Life Sciences, Shandong Agricultural University, Tai''an, Shandong, 271018, China;2. Department of Developmental Genetics, National Institute of Genetics, Mishima, Shizuoka, 411-8540, Japan;1. Department of Biology, Lund University, 22362 Lund, Sweden;2. Department of Neurobiology, Northwestern University, Evanston, IL 60208, USA;3. Skirball Institute of Biomolecular Medicine, Neuroscience Institute, Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA;1. Université Paris-Saclay, CNRS, IRD, UMR Évolution, Génomes, Comportement et Écologie, 91198 Gif-sur-Yvette, France;2. Université Paris-Saclay, AgroParisTech, 91123 Palaiseau Cedex, France |
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| Abstract: | Kraken, a novel Drosophila gene isolated from a 4–8-h-old Drosophila embryo cDNA library, shows homology to a family of serine hydrolases whose common feature is that they all catalyse breakage of substrates with a carbonyl-containing group. It is a single-copy gene with at least two introns and maps to position 21D on polytene chromosomes. kraken is a member of a conserved gene family. Messenger RNA of kraken is expressed ubiquitously in early embryogenesis. Later, it is concentrated in the foregut and the posterior midgut primordium. Towards the end of embryogenesis, expression of kraken is confined to the gastric caeca. During the third-instar larval stage, kraken is expressed at low levels in the gastric caeca and parts of the gut, and at higher levels in the fat body. We suggest a role for Kraken in detoxification and digestion during embryogenesis and larval development. |
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