Isolation and characterisation of smallminded,a Drosophila encoding a new member of the Cdc48p/VCP subfamily of AAA proteins |
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| Institution: | 1. Regional Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, 656 53 Brno, Czech Republic;2. University of Edinburgh, Institute of Genetics and Molecular Medicine, Edinburgh, Scotland EH4 2XR, United Kingdom;3. St Andrews University, St Andrews, Scotland, United Kingdom;4. Bioinformatics Institute, Agency for Science, Technology and Research (A*STAR), 30 Biopolis Street, Matrix 07-01 138671, Singapore;5. School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore;6. Department of Biological Sciences, National University of Singapore, 14, Science Drive 4, 117543, Singapore;7. University of Gdansk, International Centre for Cancer Vaccine Science, ul. Wita Stwosza 63, 80-308 Gdansk, Poland;8. University of Edinburgh, Institute of Quantitative Biology, Biochemistry and Biotechnology, Edinburgh, Scotland EH9 3BF, United Kingdom;1. Department of Pathology and Molecular Medicine, Queen''s University, Kingston, Ontario, Canada;2. Cancer Research Institute, Queen''s University, Kingston, Ontario, Canada;3. Department of Medical Sciences, Thrombosis and Atherosclerosis Research Institute, McMaster University, Hamilton, Ontario, Canada;4. Department of Medicine, Thrombosis and Atherosclerosis Research Institute, McMaster University, Hamilton, Ontario, Canada;5. Department of Medicine, Queen''s University, Kingston, Ontario, Canada |
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| Abstract: | Smallminded (smid) encodes a new member of the cdc48p/VCP subfamily of AAA proteins in Drosophila. The gene was isolated by plasmid rescue from a GAL4 enhancer trap line which shows reporter gene expression in neuroblasts, imaginal disks and a subset of sensory neurons. Larvae homozygous for the insert arrest development as second instar larvae and die without pupating. The most obvious defect in these larvae is a significantly reduced CNS, hence the naming of the gene as smallminded. The deduced amino acid sequence of smid contains a tandem duplication of the AAA nucleotide binding domain characteristic of the cdc48p/VCP subfamily. Overall, smid shares 33% identical residues with its closest relative, yeast L0919-chrXII and 26–29% with other members of the cdc48p/VCP subfamily. The most highly conserved regions of the predicted protein structure are found in and around the nucleotide binding domains. The gene is expressed at all developmental stages. |
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