cDNA cloning and developmental expression of the porcine homologue of WT1 |
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| Affiliation: | 1. Department of Pathology, Faculty of Medicine, University of Tokyo, Tokyo, Japan;2. Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Tokyo, Japan;1. Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, 91125, USA;2. Howard Hughes Medical Institute;1. State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. John F. Hardesty, MD, Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St. Louis, MO 63110, USA;4. Hefei National Laboratory for Physical Sciences, at the Microscale, Neurodegenerative Disorder Research Center, CAS Key Laboratory of Brain Function and Disease, School of Life Sciences, University of Science and Technology of China, Hefei 230026, China;5. Lunenfeld Tanenbaum Research Institute, Mt Sinai Hospital, Sinai Health Systems, Department of Ophthalmology and Vision Science, and Department of Lab Medicine and Pathobiology, University of Toronto, Toronto, ON M5G 1X5, Canada;6. CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China;7. Department of Ophthalmology, Wilmer Eye Institute Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;8. Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;9. Obstetrics and Gynecology Medical Center of Severe Cardiovascular of Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China;10. Departments of Ophthalmology and Physiology, Weill Institute for Neurosciences, University of California, San Francisco, San Francisco, CA 94143, USA;11. Department of Oncology, Division of Biostatistics and Bioinformatics, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;12. McKusick-Nathans Institute for Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;13. Institute for Data Intensive Engineering and Science, Johns Hopkins University, Baltimore, MD 21218, USA;14. Institute for Computational Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;15. Mathematical Institute for Data Science, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;16. Department of Applied Mathematics and Statistics, Johns Hopkins University, Baltimore, MD 21218, USA;17. Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;18. Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21218, USA;19. Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;20. Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China;21. Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;22. Center for Human Systems Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;23. Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;24. Beijing Institute for Brain Disorders, Beijing 100069, China;25. Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO 63110, USA;1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China;2. Fujian Provincial Key Laboratory of Refrigeration and Conditioning Aquatic Products Processing, Xiamen 361022, China;3. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China;4. Nanjing Xianou Instruments Manufacture Co., Ltd, Nanjing 210046, China;5. Fujian Anjoyfood Share Co. Ltd., Xiamen 361022, China;1. Department of Diagnostic Pathology, Ota Memorial Hospital, SUBARU Health Insurance Society, Gunma, 373-8585, Japan;2. Department of Obstetrics and Gynecology, Ota Memorial Hospital, SUBARU Health Insurance Society, Gunma, 373-8585, Japan;1. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;2. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China;3. College of Life Sciences, Nanjing Normal University, Nanjing 210023, China;4. Key Laboratory for Sustainable Utilization of Open-sea Fishery, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China |
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| Abstract: | Wilms' tumors occur most frequently in swines as sporadic tumors. To clarify the role of WT1 gene in the genesis of Wilms' tumors and genitourinary development, we have isolated the porcine homologue of the human WT1 gene (pWT1) and analyzed its expression in various organs including the kidney. The open reading frame of pWT1 cDNA was extremely homologous to the human counterpart: 94% identical at the nucleotide level and 98% at the polypeptide level. In particular, the zinc finger region was more than 97% similar to human WT1 gene at the nucleotide level and 100% at the polypeptide level. pWT1 mRNA was found to be expressed in new-born kidney, spleen, testis, and embryonic kidneys, suggesting a possible association of pWT1 with the development of the genitourinary system. In conclusion, the nucleotide sequence and expression patterns in organs of pWT1 were similar to those of human WT1. Therefore, swines could provide good models for analyzing the contributions of WT1 gene to genitourinary development and genesis of Wilms' tumors. |
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