Steroid metabolism in granulosa and theca interna cells from preovulatory follicles of domestic hen (Gallus domesticus) |
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| Institution: | 1. Department of Embryology, School of Medicine, National Autonomous University of Mexico, P.O. Box 70-159, Mexico City, D.F., 04510, Mexico;2. Department of Cellular and Tissular Biology, School of Medicine, National Autonomous University of Mexico, P.O. Box 70-159, Mexico City, D.F., 04510, Mexico;3. Department of Biochemistry, School of Medicine, National Autonomous University of Mexico, P.O. Box 70-159, Mexico City, D.F., 04510, Mexico;1. Department of Chemistry, CMS College, Kottayam, Kerala, India;2. Department of Anatomy, Government Medical College, Kottayam, India;3. International and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, 686 560, Kerala, India;4. School of Pure and Applied Physics, Mahatma Gandhi University, Kottayam, 686 560, Kerala, India;5. Department of Chemistry, Bishop Moore College, Mavelikkara, Kerala, India;6. School of Chemical Sciences, Mahatma Gandhi University, Kottayam, 686 560, Kerala, India;3. Laboratory of Immunology and Microbial Pathogenesis, National Institute of Animal Biotechnology, Hyderabad, Telangana 500032, India and;4. Graduate Studies, Manipal Academy of Higher Education, Manipal, Karnataka 576104, India;1. Department of Mechanical and Aerospace Engineering, ADAMUS Laboratory, University of Florida, 939 Sweetwater Dr., Gainesville, FL 32611 – 6250, USA;2. Department of Mechanical and Aerospace Engineering, Sapienza University of Rome, via Eudossiana 18, 00184, Italy;3. Department of Aeronautics and Astronautics Engineering, Stanford Space Rendezvous Lab, Stanford University, 496 Lomita Mall, Stanford, CA 94305-4035, USA;2. Anhui province key laboratory of local livestock and poultry genetic resource conservation and bio-breeding, Anhui Agricultural University, Hefei 230036, P.R. China;3. College of biological and pharmaceutical engineering, West Anhui University, Liuan 237012, China |
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| Abstract: | The capability of granulosa and theca interna cells, from preovulatory follicles of the domestic hen, to metabolize steroid precursors was evaluated. Granulosa and theca interna cells were isolated from ovarian preovulatory follicles at three different developmental stages: F1, F3 and F5. Tritiated pregnenolone (P5), progesterone (P4), dehydroepiandrosterone (DHEA), androstenedione (A4) and testosterone (T) were employed as precursors and their metabolic products were evaluated. The major metabolite of P5 by granulosa cells was P4, but we also observed low amounts of 5β-pregnandione. DHEA metabolism by granulosa cells yielded mainly A4, and minute quantities of 5β-androstan-3,17-dione (5β-dione) were detected. The only significant metabolite obtained in granulosa cells from A4 was 5β-dione, whereas T was only transformed into A4. On the other hand, P5 metabolism by theca interna cells yielded A4 as the main product, also P4, 17α-OHP4, 17α-OHP5, 5β-pregnandione, and DHEA, were found. When DHEA was the precursor A4 was produced in higher amounts than 5β-dione. A4 was mainly transformed into 5β-dione. In similar conditions, T was transformed into A4. These results show that granulosa cells have enzymatic activities of 3β-hydroxysteroid dehydrogenase/5-4 isomerase (3β-HSD from P5 and DHEA), 17β-hydroxysteroid dehydrogenase (17β-HSD from T) and 5β-reductase (from P5, DHEA and A4). Whereas theca interna cells have enzymatic activities of cytochrome P450c17 (from P5 and P4), 3β-HSD (from P5 and DHEA), 17β-HSD (from T) and 5β-reductase (from P4, DHEA and A4). These data support the concept that theca interna cells have the ability to synthesize androgens from progestins produced in granulosa cells. In addition, since theca interna cells did not show the capacity to aromatize androgens suggests that interaction between theca interna and theca externa cells occurs in vivo, thus confirming the three cell model for estrogen production. Furthermore, the fact that other metabolites were produced both in granulosa and theca interna cells, but in a different extent, suggests that complex mechanisms are participating in the regulation of steroid synthesis in avian ovary follicles. |
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