Prediction of porcine semen fertility by homologous in vitro penetration (hIVP) assay |
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| Institution: | 1. Laboratory of Biology of Reproduction, Institute of Biomedical Sciences, Federal University of Uberlândia, CEP 38400-902, Uberlândia, MG, Brazil;2. Faculty of Computer Science, Federal University of Uberlândia, CEP 38400-902, Uberlândia, MG, Brazil;1. Department of Animal Genetics and Breeding, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141004, Punjab, India;2. Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141004, Punjab, India;1. Clinic for Reproduction and Large Animals, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia;2. Small Animal Clinic, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia;3. Institute for Preclinical Sciences, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, Ljubljana, Slovenia;1. Institute of Animal Health and Cattle Development (INDEGSAL), University of León, 24071, León, Spain;2. Institute of Biomedicine (IBIOMED), University of León, 24071, León, Spain;3. Department of Clinical and Experimental Medicine (IKE). BKH, Obstetrics and Gynecology, Linköping University, 58183, Linköping, Sweden;4. Department of Animal Medicine, Surgery and Anatomy (Animal Medicine and Surgery), University of León, 24071, León, Spain;5. Division of Reproduction, Swedish University of Agricultural Sciences, 75007, Uppsala, Sweden;6. Dept. of Molecular Biology (Cell Biology), University of León, León, Spain |
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| Abstract: | A field trial was conducted to compare the fertility predicting capacity of different sperm assays applying classical semen analysis, sperm function and the homologous in vitro penetration test (hIVP) to 60 ejaculates from four boars collected over a period of 15 weeks. No differences were found between the groups of fertility (Low Fertility: <20%; Intermediate: 40–60% and High: >80%) for sperm-rich fraction volume collection, sperm concentration, total sperm number, cationic contents in seminal plasma and ATP concentration. Partial differences were found in the parameters of motility, normal morphology, normal apical ridge (NAR), viability with eosin–nigrosin stain, hypo-osmotic swelling test (HOS), osmotic resistance test (ORT) and functional membrane integrity (with carboxyfluorescein diacetate, DCF). These parameters would be useful for detecting sperm with poor fertility, but they are not precise enough to discriminate an ejaculate with higher fertility than the herd median. Only the penetration percentage (10.24±1.45 vs. 55.13±3.35 vs. 84.72±1.73) and sperm number per oocyte (1.29±0.07 vs. 11.29±1.79 vs. 25.86±1.43) in a hIVP system were parameters with a predictive capacity to discriminate between the three fertility groups. Consequently, hIVP was found to be the best seminal assay and it may improve the in vitro assessment of sperm fertilizing ability. |
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