Cloning and characterization of the gene encoding beta subunit of mitochondrial processing peptidase from the basidiomycete Lentinula edodes |
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Affiliation: | 1. School of Xiangya Pharmaceutical Sciences, Central South University, 172 Tong Zi Po Road, Changsha 410013, China;2. Medical College, Hunan Normal University, 371 Tong Zi Po Road, Changsha 410006, China;3. Changsha Duxact Biotech Co., Ltd., C10 Building, Lugu S&T Park, 28 Lutian Rd, Changsha 411000, China;1. Campbell Research Institute, Centre for Addiction and Mental Health, Toronto, Ontario M5T 1R8, Canada;2. Medical and Forensic College, Xi''an Jiaotong University Health Science Center, Xi''an, Shaanxi, 710061, PR China;3. Department of Psychiatry, University of Toronto, Toronto, Ontario M5T 1R8, Canada |
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Abstract: | We have isolated the gene encoding the beta subunit of mitochondrial processing peptidase (β-MPP) from the shiitake mushroom Lentinula edodes. It is a nuclear gene with two small introns. Comparison with known β-MPP genes revealed that the L. edodes gene is most closely related with that from Neurospora crassa, with 60.8% identities and 87% similarity in the amino-acid sequences. The deduced L. edodes β-MPP peptide sequence contains the inverse zinc-binding motif (H–X–E–H) that has been found in a large family of zinc-binding metalloproteinases including bacterial proteinases, insulin degrading enzymes and β-MPPs. The two histidines are thought to contribute two of the three residues for zinc binding. The expression of L. edodes β-MPP is higher during the development of the fruiting bodies, suggesting that higher mitochondrial activities may be required to meet the energy demand in the rapid growth of the fruiting bodies. |
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