Existence of a bovine LINE repetitive insert that appears in the cDNA of bovine protein BCNT in ruminant,but not in human,genomes |
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| Institution: | 1. National Institute of Infectious Diseases, Tokyo 162, Japan;2. Mitsubishi Kasei Institute of Life Sciences, Tokyo 194, Japan;3. Institute for Virus Research, Kyoto University, Kyoto 606-01, Japan;4. Tokyo Institute of Technology, Yokohama 226, Japan;5. National Institute of Health Sciences, Tokyo 158, Japan;1. MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Crewe Road South, Edinburgh EH4 2XU, UK;2. Northern Institute for Cancer Research, Framlington Place, Medical Faculty, Newcastle upon Tyne NE2 4HH, UK;3. Centre for Cardiovascular Science, Queen’s Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, UK;1. Research Center for High Altitude Medicine, Qinghai University Medical College, 810001 Qinghai, Xining, PR China;2. Key Laboratory for Application of High Altitude Medicine in Qinghai Province, Qinghai University Medical College, 810001 Qinghai, Xining, PR China;3. Qinghai-Utah Joint Research Key Lab for High Altitude Medicine, Qinghai University Medical College, 810001 Qinghai, Xining, PR China;4. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;5. Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;6. Departments of Pediatrics, Medicine, Oncology, Radiation Oncology, and Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;1. National Engineering Laboratory of Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction, MARA, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China;2. College of Animal Science, Xinjiang Agricultural University, Urumqi, 830052, China;3. Department of Animal Sciences, Purdue University, West Lafayette, IN 47907;4. Beijing Sunlon Livestock Development Company Limited, 10029, Beijing, China;1. Key Laboratory for Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China;2. Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, China |
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| Abstract: | A novel protein, BCNT, originally isolated from bovine brain and named after Bucentaur, contains an internal portion that is translated from part of bovine LINE repetitive sequence (Bov-B LINE). Human cDNA highly homologous to the bovine bcnt (bbcnt) cDNA has been isolated but does not contain a sequence similar to the Bov-B LINE insert (Nobukuni, T., Kobayashi, M., Omori, A., Ichinose, S., Iwanaga, T., Takahashi, I., Hashimoto, K., Hattori, S., Kaibuchi, K., Miyata, Y., Masui, T., Iwashita, S., 1997. An Alu-linked repetitive sequence corresponding to 280 amino acids is expressed in a novel bovine protein, but not in its human homologue. J. Biol. Chem. 272, 2801–2807). In this study, we conducted a polymerase chain reaction analysis to investigate whether such a Bov-B LINE insert is present in bcnt orthologs in other animals and in the genomic sequence of the human BCNT (hBCNT) gene. The results indicate that the Bov-B LINE insert is present in the genomic sequences of bcnt orthologs from sheep, goats, axis deer, and mouse deer (chevrotin), that is in Ruminantia, but not in pigs or human. Analysis of the bbcnt genomic sequence around the Bov-B LINE insert revealed a large part of the inserted Bov-B LINE sequence to be included in an exon; this is followed by a 54-nucleotide sequence that is highly homologous to Bov-B LINE in the 3′-side intron. The hBCNT gene was isolated and found to consist of seven exons and six introns, among which the intron corresponding to the Bov-B LINE insertion site in the bbcnt genome is 16.5 kb in length with no sequence similar to Bov-B LINE. Based on these results, it seems likely that the Bov-B LINE insert is derived from a long Bov-B LINE repetitive sequence transposed to an ancestral bcnt gene in Ruminantia and reformed as a new exon through new splicing sites in the transposed sequence. |
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