Distribution of alpha 1 subunit isoform of (Na,K)-ATPase in the rat spinal cord |
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Authors: | Scott T Sayers PhD Talat Khan Ramzan Shahid Michael F Dauzvardis George J Siegel |
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Institution: | (1) Rehabilitation Research and Development Center, Hines VA Hospital, 60141 Hines, IL;(2) Neurology Service, Hines VA Hospital, 60141 Hines, IL;(3) Department of Neurology, Loyola University Medical School, 60153 Maywood, IL;(4) Rehabilitation Research and Development 151L, Neuro-Regeneration Laboratory, Department of Veterans Affairs, The Edward Hines, Jr. Hospital, 60141 Hines, IL |
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Abstract: | Three isoforms of the alpha subunit of (Na,K)-ATPase have been identified in the rat central nervous system. Using a probe specific for the alpha 1 isoform, mRNA levels were measured from five sections of the rat spinal cord using slot blot techniques. Assigning a value of 1 to the slope obtained from the cervical section, the upper thoracic section was 2.6 times higher; the midthoracic section was 4.5 times higher; the lower thoracic section was 2.6 times higher; and the lumbar section was 1.7 times higher. The results suggest that alpha 1 isoform mRNA levels are not uniform throughout the spinal cord. In situ hybridization techniques showed that alpha 1 isoform mRNA was diffusely abundant in glial and central canal ependymal cells, while labeled neurons were localized exclusively in lateraily located anterior horn neurons in cervical, thoracic, and lumbar segments and in ventromedial neurons in mid-thoracic spinal cord. Also, dorsal root ganglia neurons were extensively labeled at all segments.Special issue dedicated to Dr. Bernard W. Agranoff. |
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Keywords: | (Na K)-ATPase spinal cord slot blots in situ hybridization |
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