Characterization of Sulphate Uptake in Anacystis nidulans |
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Authors: | HANS CHRISTIAN UTKILEN MIKAL HELDAL GJERT KNUTSEN |
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Institution: | Institut für Angewandte Botanik der Universität Münster, D-4400 Münster, West-Germany |
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Abstract: | Sulphate uptake in the blue-green alga Anacystis nidulans appears based upon an active mechanism with a Km of 0.75 μM and Vmax of 0.7 pmol/min × 106 cells. Sulphate uptake is competitively inhibited by thiosulphate and sulphite. The sulphate uptake has a pH optimum at 8 and a temperature optimum at 40°C. By increasing the extracellular sulphate concentration from 0.1 to 10 μM the sulphate pool in Anacystis was altered from 8.3, 10?5M to 5.9, 10?4M. |
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