| 油松胚珠愈伤组织诱导和悬浮细胞系的建立 |
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| 引用本文： | 杨远媛,贺窑青,郑彩霞.油松胚珠愈伤组织诱导和悬浮细胞系的建立[J].植物生理学通讯,2005,41(5):591-594. |
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| 作者姓名： | 杨远媛 贺窑青 郑彩霞 |
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| 作者单位： | 北京林业大学生物科学与技术学院,北京,100083 |
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| 摘 要： | 雌配子体处于游离核时期的油松胚珠以含不同浓度和配比生长调节剂的培养基诱导产生愈伤组织,并建立了悬浮细胞系.这一悬浮细胞系生长快,分散性好,稳定均一,适用于研究其生理、生化和细胞周期调控.
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| 关 键 词： | 油松胚殊 愈伤组织 悬浮细胞系 |
| 收稿时间： | 2005-02-05 |
| 修稿时间： | 2005-06-20 |
Induction of Callus from the Ovule of Pinus tabulaeformis Carr and Establishment of Suspension Cell Line |
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| YANG Yuan-Yuan,HE Yao-Qing,ZHENG Cai-Xia.Induction of Callus from the Ovule of Pinus tabulaeformis Carr and Establishment of Suspension Cell Line[J].Plant Physiology Communications,2005,41(5):591-594. |
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| Authors: | YANG Yuan-Yuan HE Yao-Qing ZHENG Cai-Xia |
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| Institution: | College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China |
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| Abstract: | Ovule callus of Pinus tabulaeformis Carr, which female gametophytes were on the mitosis of the free nuclei, was induced on MS media with different concentrations and combinations of hormones and the suspension cell line was established. The suspension cell line grew rapidly and was fairly homogenous. It was suitable to be studied on the mechanisms of the plant physiology, biochemistry and the regulation of cell cycle. |
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| Keywords: | ovule of Pinus tabulaeformis Carr callus suspension cell line |
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