Binding and incorporation of lecithin-cholesterol vesicles to lymphocytes: A spin-label study |
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| Authors: | George Dresdner Lennart Hammarström C I Edvard Smith |
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| Institution: | (1) Department of Biophysics, Stockholm University, Arrhenius Laboratory, S-106 91 Stockholm, Sweden;(2) Department of Immunobiology, Karolinska Institute, Wallenberg Laboratory, S-104 05 Stockholm, Sweden;(3) Present address: The Gustaf Werner Institute, Uppsala University, Box 531, S-751 21 Uppsala, Sweden |
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| Abstract: | Summary When lecithin-cholesterol vesicles, containing the membrane-bound spin probe 3-doxyl-cholestane, were set in contact with mouse lymphocytes, the vesicles adsorbed to the cell and vesicle-membrane components were transferred to it. The spin probe was enzymatically reduced at the inside of the cell membrane. The spin-label method provided a means to determine quantitatively the extent of vesicles adsorption and vesicle-cell fusion by measuring the transfer of vesicles membrane material to the cell. This method, together with the reduction of spin label by the cell, allowed also a quantitative estimate of the extent of endocytosis during cell-liposome interaction. |
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| Keywords: | lymphocyte-liposome interaction vesicle-cell fusion endocytosis cellular vesicle adsorption membrane-bound spin probe cholestane spin label |
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