Efficiency of the inbreeding coefficient f and other estimators in detecting null alleles,as revealed by empirical data of locus oke3 across 65 populations of chum salmon Oncorhynchus keta |
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| Authors: | L. A. Zhivotovsky E. G. Shaikhaev G. A. Rubtsova K. I. Afanasiev M. V. Shitova G. O. Shaikhaev A. J. Gharrett |
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| Affiliation: | 1. N.I. Vavilov Institute of General Genetics, Moscow, Russia;2. Isogene Lab Ltd., Moscow, Russia;3. School of Fisheries and Ocean Sciences, University of Alaska Fairbanks, Juneau, AL, U.S.A |
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| Abstract: | A survey of 65 populations of chum salmon Oncorhynchus keta across the species range revealed homozygote excess (947 homozygotes in 2954 fish) at a polymerase chain reaction (PCR)‐based simple sequence repeat (SSR) locus oke3 with multiple alleles, whereas re‐designed PCR primers indicated that 328 of these homozygotes were actually heterozygotes. Statistically significant high positive values of inbreeding coefficients, f, in multiple populations appeared to be a reliable predictor of null alleles. Based on these data, three methods were checked for their ability to estimate null‐allele frequencies. |
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| Keywords: | DNA amplification f‐value null allele population study SNP |
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