Antibody detection of SARS-CoV spike and nucleocapsid protein |
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| Authors: | Chang Mau-Sun Lu Yen-Ta Ho Shin-Tsung Wu Chao-Chih Wei Tsai-Yin Chen Chia-Ju Hsu Yun-Ting Chu Po-Chen Chen Ching-Hsin Chu Jien-Ming Jan Ya-Lin Hung Chia-Chien Fan Chi-Chen Yang Yuh-Cheng |
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| Institution: | Department of Medical Research, Mackay Memorial Hospital, ROC, Taipei, Taiwan. |
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| Abstract: | Early detection and identification of SARS-CoV-infected patients and actions to prevent transmission are absolutely critical to prevent another SARS outbreak. Antibodies that specifically recognize the SARS-CoV spike and nucleocapsid proteins may provide a rapid screening method to allow accurate identification and isolation of patients with the virus early in their infection. For this reason, we raised peptide-induced polyclonal antibodies against SARS-CoV spike protein and polyclonal antibodies against SARS-CoV nucleocapsid protein using 6x His nucleocapsid recombinant protein. Western blot analysis and immunofluorescent staining showed that these antibodies specifically recognized SARS-CoV. |
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| Keywords: | SARS Spike Nucleocapsid |
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