首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Oxygen transfer correlation in high cell density culture of recombinant E. coli
Authors:Chul Soo Shin  Min Sun Hong  Jeewon Lee
Institution:(1) Biochemical Engineering Laboratory, Hanhyo Institutes of Technology, San 6, Siheung-City, Kyungki-Do, South Korea
Abstract:Summary A recombinant E. coli BL21pET3a-T2M2] was cultivated in fed-batch cultures and cell mass increased to more than 70g/L. The volumetric oxygen transfer coefficient was estimated in a range of various fermentation parameters (agitation speed, oxygen flow rate and cell mass concentration) and finally the oxygen transfer correlation in bioreactor containing the recombinant E. coli cultures was determined as: kspla = 0.0195 (Pg/V)0.55 (Vs)0.64 (1+2.12X+0.20X2)–0.25.
Keywords:
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号