Increased level of superoxide dismutase (SOD) activity in larvae of Chironomus ramosus (Diptera: Chironomidae) subjected to ionizing radiation |
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| Authors: | K.D. Datkhile R. Mukhopadhyaya T.K. Dongre B.B. Nath |
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| Affiliation: | 1. Centre for Advanced Studies, Department of Zoology, University of Pune, Pune 411007, India;2. Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai - 400085, India;3. Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India;1. Dipartimento di Biologia e Biotecnologie “L. Spallanzani” Università di Pavia, via Ferrata 9, 27100 Pavia, Italy;2. Istituto di Genetica Molecolare del CNR, Sezione di Istochimica e Citometria, via Ferrata 9, 27100 Pavia, Italy;3. Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, 73100 Lecce, Italy;1. Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, Key Laboratory for Aerosol-Cloud-Precipition of China Meteorological Administration, Nanjing University of Information Science & Technology, Nanjing 210044, China;2. Laboratory for Middle Atmosphere and Global Environment Observation (LAGEO), Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100029, China;1. National Key Laboratory on Electromagnetic Environmental Effects and Electro-Optical Engineering, PLA University of Science and Technology, Nanjing, China;2. Army Aviation Institute, Beijing, China;3. PLA University of Science and Technology, Nanjing, China |
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| Abstract: | A battery of enzymes from the eukaryotic antioxidant defense system was measured in salivary gland and in whole body extract of fourth instar larvae of Chironomus ramosus with an objective of finding any clue for the dipteran insect's capacity to tolerate heavy doses of ionizing radiation. Levels of activity of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), and glutathione peroxidase (GSH-Px) were quantified in 30 days old larvae exposed to LD20 dose of gamma radiation. Compared to controls, activity of Cu,Zn-SOD increased 3 to 4 fold and catalase 2 fold in response to ionizing radiation stress, while activities of GR and GSH-Px enzymes were decreased. Among the other SOD isoenzymes, our results showed comparable levels of Mn-SOD and Cu,Zn-SOD activity in control and irradiated groups of larvae. The increase in levels of the Cu,Zn-SOD isoenzyme was also confirmed by Western blot and zymography supported by densitometric quantification. No evidence of Fe-SOD was found in C. ramosus larvae. These findings could help to explain the persistence of natural populations of Chironomus in radioactively contaminated regions. |
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