Carbofuran-induced alterations in the energy metabolism and reproductive behaviors of Hyalella castroi (Crustacea,Amphipoda) |
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| Authors: | BK Dutra FA Fernandes AL Lauffer GT Oliveira |
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| Institution: | 1. Instituto Nacional de Limnología (CONICET-UNL), Paraje El Pozo s/n, (3000) Santa Fe, Argentina;2. Escuela Superior de Sanidad, Facultad de Bioquímica y Ciencias Biológicas, UNL Paraje El Pozo s/n, (3000) Santa Fe, Argentina;3. Facultad de Ciencia y Tecnología, UADER, Corrientes y Andrés Pasos, (3100) Paraná, Entre Ríos, Argentina;1. Zoology Department, Faculty of Science, New Valley Branch, Assiut University, Assiut, Egypt;2. Zoology Department, Faculty of Science, Assiut University, Assiut, Egypt;3. Biology Department, Faculty of Science, North Jeddah, King Abdulaziz University, Jeddah, Saudi Arabia;1. Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China;2. Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China;3. College of Fisheries and Life Science, Dalian Ocean University, Dalian, PR China;1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, 510642, China;2. Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China |
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| Abstract: | Continuous or pulse exposure to pesticides may have negative effects on non-target organisms, resulting in a number of pathological and disturbed biochemical processes, including changes in energy budgets. The objective of this investigation was to examine the potential effects of carbofuran on the biochemical composition (glycogen, proteins, total lipids, triglycerides and cholesterol), levels of lipoperoxidation, Na+/K+ATPase activity, and reproductive behaviors (number of reproductive pairs, ovigerous females, and number of eggs) in the amphipod Hyalella castroi. The amphipods were collected in spring 2007, in the southern Brazilian highlands. In the laboratory, the animals were kept in aquariums under controlled conditions for 7 days, and after this period were exposed to 1 or 10 µg/L of carbofuran for 7 days. After the period of exposure, the animals were immediately frozen for determination of glycogen, proteins, lipids, triglycerides, total cholesterol, levels of lipoperoxidation, and Na+/K+ATPase activity. During each day of culture, reproductive behaviors were observed. Carbofuran induced significant decreases in biochemical reserves (glycogen, proteins, lipids, triglycerides and cholesterol), a significant increase in lipoperoxidation levels, and a decrease in Na+/K+ATPase activity in both males and females. Studies of all the biochemical parameters seem to be quite promising, in order to assess and predict the effects of toxicants on non-target organisms. The results showed that reproductive behaviors may provide sensitive criteria for assessing ecotoxicological effects. H. castroi lives among rooted aquatic macrophytes, and we suggest that it is a sensitive species that could be used in monitoring studies. |
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