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Effects of cadmium on respiratory burst,intracellular Ca2+ and DNA damage in the white shrimp Litopenaeus vannamei
Authors:Ming Chang  Wei-Na Wang  An-Li Wang  Ting-Ting Tian  Peng Wang  Ying Zheng  Yuan Liu
Institution:1. School of Animal Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Tambon Suranaree, Muang, Nakhon Ratchasima 30000, Thailand;2. Department of Biology, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand
Abstract:Acute effects of heavy metal ions on shrimp have been an area of intense study worldwide. However, the molecular mechanism by which cadmium-induced injury occurs remains largely unclear, and methods for mitigating toxicity in vivo have rarely been reported. In this study, the changes in respiratory burst and intracellular free calcium in haemocytes of pacific white shrimp, Litopenaeus vannamei, after exposure to Cd2+ (CdCl2) were examined using flow cytometry. Meanwhile, DNA damage and repair in haemocytes and hepatopancreas cells were studied using the comet assay. Respiratory burst generation, intracellular Ca2+ concentration (Ca2+]i) and DNA damage in haemocytes and hepatopancreas cells all exhibited a dose-dependent increase and a time-dependent change after treatment with Cd2+ compared with controls. These results indicate that Cd can induce oxidative stress and DNA damage in the shrimp L. vannamei. Moreover, the results also demonstrate that these parameters can be used as sensitive indicators of exposure to this genotoxicant.
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