Structure and chain conformation of a (1 → 6)-α-d-glucan from the root of Pueraria lobata (Willd.) Ohwi and the antioxidant activity of its sulfated derivative |
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| Authors: | Hengxiang Cui Qin Liu Yongzhen Tao Hongfeng Zhang Lina Zhang Kan Ding |
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| Institution: | aSchool of Life Science, East China Normal University, Shanghai 200062, China;bGlycochemistry & Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong zhi Road, Pudong, Shanghai 201203, China;cDepartment of Chemistry, Wuhan University, Wuhan 430072, China |
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| Abstract: | A water soluble glucan, PLB-2C, was isolated from the water extract of the root of Pueraria lobata (Willd) Ohwi using anion-exchange and gel permeation chromatography. Its structure was investigated by gas chromatography (GC), gas chromatography–mass spectrometry (GC–MS), infrared (IR) spectra, and nuclear magnetic resonance (NMR) spectroscopy of heteronuclear single quantum coherence (HSQC) and heteronuclear multiple bond correlation (HMBC) techniques. The results indicated that PLB-2C was a linear glucan composed of (1 → 6)-α-d-Glcp. Chain conformation study showed that the polysaccharide took random coil compact conformation. In vitro cell viability assay by MTT method, its sulfated derivative PLB-2CS which was substituted at 2-O, 3-O, 4-O positions, at 0.1, 1, and 5 mg/ml, could attenuate PC12 cell damage significantly caused by hydrogen peroxide. |
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| Keywords: | Pueraria lobata (1 → 6)-α d-Glucan" target="_blank">-d-Glucan Chain conformation Antioxidant effect Sulfated polysaccharide |
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