波尔山羊9个微卫星标记与产羔性状的关联研究

选择与绵羊高繁殖力主效基因FecB和FecX紧密连锁的5个微卫星标记BM1329, BM143, OarHH55, TGLA68和LSCV043, 和其他4个微卫星标记ETH225, INRA063, BM1225和MAF0214, 分析研究其与波尔山羊产羔数的关联。结果表明: 所研究的9个波尔山羊微卫星基因座均为高度多态性基因座(PIC > 0.5)。找到与波尔山羊产羔性状显著相关的等位基因计12个。其中与波尔山羊第一胎产羔数有显著正效应的等位基因3个: BM143的120 bp和108 bp, ETH225的183 bp; 与波尔山羊第一胎产羔数有显著负效应的等位基因8个: BM1329的216 bp、BM143的110 bp、BM1225的255 bp和239 bp、INRA063的175 bp、177 bp和189 bp, ETH225的163 bp。与波尔山羊第二胎产羔数有显著正效应的等位基因1个: TGLA68的115 bp。

Correlation analysis between 9 microsatellite markers and fecundity trait of Boer goat

Five microsatellite markers BM1329, BM143, OarHH55, TGLA68, and LSCV043, which were closely linked to the fecundity gene FecB and FecX in ovis aries sheep, were chosen based on their high conservatism in the close species and another 4 microsatellite markers ETH225, INRA063, BM1225, and MAF0214 were selected to analyze their correlation with the litter size of Boer goat. All the 9 microsatellite loci in Boer goat were high polymorphism locus (PIC > 0.5). Twelve alleles were found to be significantly correlate with litter size of Boer goat. Three of them, i.e., 120 bp and 108 bp of BM143, 183 bp of ETH225, were positively correlated to first parity litter size of Boer goat. Eight alleles, i.e., 216 bp of BM1329, 110 bp of BM143, 255 bp and 239 bp of BM1225, 175 bp, 177 bp and 189 bp of INRA063, and 163 bp of ETH225, had negative correlation with first parity litter size. One allele, i.e., 115 bp of TGLA68, were positively correlated to second parity litter size.