Plant regeneration from protoplasts of embryogenic cell suspensions of Coffea arabica L. cv. caturra |
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Authors: | Jose Ricardo Acuna Myriam de Pena |
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Institution: | (1) Laboratorio de Investigaciones sobre la Quimica del Cafe (LIQC), A.A. 3937, Bogota, Colombia |
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Abstract: | Coffee plants were regenerated from protoplasts isolated from embryogenic cell suspension cultures derived from somatic embryos of Coffea arabica L. cv. caturra. Yields of viable protoplasts ranged from 1×105 to 6×105 protoplast/g fresh weight. Protoplast preparations usually contained no contaminating cells, and when present, the number of cells never exceeded 0.1% of the total. Plating efficiencies of protoplast ranged from 1 to 10%. Embryogenic protocolonies obtained after several subcultures in a medium supplemented with 0.5 mg/l each of benzylaminopurine, 2,4-dichlorophenoxyacetic acid and naphtaleneacetic acid, were transferred to a medium lacking plant growth regulators. Well differentiated embryos were formed in selected protocolonies that contained many embryos-like structures. Approximately 70% of the somatic embryos developed into green rooted plantlets which were succesfully transferred to vessels containing sterilized scoria. Plants grown for two months in scoria were finally transferred to greenhouse.Abbreviations B5
medium according to Gamborg et. al.(1968)
- BAP
6-benzylaminopurine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- NAA
naphtaleneacetic acid |
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Keywords: | embryogenic cell suspension protoplast regeneration coffee (Coffea arabica L cv caturra) |
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