Probing the structure-activity relationship of Escherichia coli LT-A by site-directed mutagenesis |
| |
Authors: | Mariagrazia Pizza Mario Domenighini Wim Hol Valentine Giannelli Maria Rita Fontana Marzia M Giuliani Claudia Magagnoli Samuele Peppoloni Roberto Manetti Rino Rappuoli |
| |
Institution: | Immunobioiogical Research Institute Siena (IRIS), Via Fiorentina 1, 53100 Siena, Italy.;Department of Biological Structures, School of Medicine, University of Washington, Seattle, WA 98195, USA. |
| |
Abstract: | Computer analysis of the crystallographic structure of the A subunit of Escherichia coil heat-labile toxin (LT) was used to predict residues involved in NAD binding, catalysis and toxicity. Following site-directed mutagenesis, the mutants obtained could be divided into three groups. The first group contained fully assembled, non-toxic new molecules containing mutations of single amino acids such as Val-53 → Glu or Asp, Ser-63 → Lys, Val-97 → Lys, Tyr-104 → Lys or Asp, and Ser-14 → Lys or Glu. This group also included mutations in amino acids such as Arg-7, Glu-110 and Glu-112 that were already known to be important for enzymatic activity. The second group was formed by mutations that caused the collapse or prevented the assembly of the A subunit: Leu-41 → Phe, Ala-45 → Tyr or Glu, Val-53 → Tyr, Val-60 → Gly, Ser-68 → Pro, His-70 → Pro, Val-97 → Tyr and Ser-114 → Tyr. The third group contained those molecules that maintained a wild-type level of toxicity in spite of the mutations introduced: Arg-54 → Lys or Ala, Tyr-59 → Met, Ser-68 → Lys, Ala-72 → Arg, His or Asp and Arg-192 → Asn. The results provide a further understanding of the structure–function of the active site and new, non-toxic mutants that may be useful for the development of vaccines against diarrhoeal diseases. |
| |
Keywords: | |
|
|