| 毛豆根瘤菌转吸氢基因的研究 |
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| 引用本文： | 龙敏南,张凤章,许良树.毛豆根瘤菌转吸氢基因的研究[J].亚热带植物科学,1996,25(1):13-16. |
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| 作者姓名： | 龙敏南 张凤章 许良树 |
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| 作者单位： | 厦门大学生物系, 厦门 361005 |
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| 摘 要： | 通过三亲本杂交将携带豌豆根瘤菌吸氢基因的质粒pAL618转移到台湾毛豆根瘤菌292-C中,经抗性筛选、质粒检测和吸氢活性测定,得到能稳定遗传的结合株292-C2和292-C3。在自生条件下结合株均表现较高的吸氢活性,而受体株292-C不吸氢,在共生条件下结合株表现为高吸氢,而受体株表现为放氢。
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| 关 键 词： | 台湾毛豆根瘤菌 吸氢基因 吸氢活性 |
| 收稿时间： | 1995-12-27 |
Translation of Hydrogen Uptake Genes into Rhizobium japonicum |
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| Long Minnan,Zhang Fengzhang,Xu Liangshu.Translation of Hydrogen Uptake Genes into Rhizobium japonicum[J].Subtropical Plant Science,1996,25(1):13-16. |
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| Authors: | Long Minnan Zhang Fengzhang Xu Liangshu |
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| Institution: | Department of Biology, Xiamen University, Xiamen 361005 |
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| Abstract: | The cosmid pAL618 containing the hydrogen uptake genes of Rhizobium leguminosarum was transferred into Rhizobium Japonicum (Glycine max meer 292-C) by triparent mating system,Two transconjugants 292-C2 and 292-C3 were selected in Rhizobium mininal medium containing Ampicillin (25 mg/L) and Tetraeycline (10 mg/L).The expression of hydrogenase activities of transconjugants were studied.The transconju-gants 292-C2 and 292-C3 showed high hydrogenase activity both in free-living and symbi-otic state whiie the recipient showed no hydrogen uptake activity in free-living state and hydrogen evolution in symbiotic state. |
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| Keywords: | Rhizobium japonicum Hydrogen uptake genes Hydrogenase activity |
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