Over-expression and characterization of the recombinant small heat shock protein from Pyrococcus furiosus |
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Authors: | Huayou Chen Zhongmei Chu Yi Zhang Shengli Yang |
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Institution: | (1) Shanghai Institutes for Biological Sciences, Chinese Academy of Science, 200233 Shanghai, China;(2) Graduate School of Chinese Academy of Science, 100049 Beijing, China |
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Abstract: | The gene encoding a small heat shock protein (sHSP) from Pyrococcus furiosus was redesigned and chemically synthesized by using bacteria-preferred codons. The gene product was over-expressed in Escherichia coli BL21(DE)3 and purified to homogeneity. In the presence of this protein, the activities of Taq DNA polymerase, DNA restriction endonuclease HindIII and lysozyme were protected at elevated temperature, and also, thermal aggregation of lysozyme was prevented by this purified recombinant sHSP.Huayou Chen, Zhongmei Chu, Contributed equally to this work |
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Keywords: | Chaperone Pyrococcus furiosus Small heat shock protein |
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