Fructanolytic and saccharolytic enzymes of Treponema zioleckii strain kT |
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| Authors: | A Kasperowicz P Prista? M Piknová P Javorský W Guczyńska T Micha?ovski E Kwiatkowska |
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| Institution: | 1. Institute of Animal Physiology, Slovak Academy of Science, Soltesovej 4, Ko?ice, Slovakia;2. The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, ul. Instytucka 3, 05-110 Jablonna near Warsaw, Instytucka Street 3, Poland;3. From the Department of Biological Sciences and the Purdue Center for Cancer Research and;4. the Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907;1. United States Department of Agriculture, Agricultural Research Service, Albany, CA 94710 USA;2. United States Department of Agriculture, Agricultural Research Service, U.S. Arid Lands Agricultural Research Center, Maricopa, AZ 85138 USA;3. United States Department of Agriculture, Agricultural Research Service, Office of National Programs, Crop Production and Protection, Beltsville, MD 20705 USA |
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| Abstract: | Enzymes in the newly described rumen bacterium, Treponema zioleckii strain kT, capable of digesting Timothy grass fructan, inulin, and sucrose were identified and characterized. Two specific endolevanases and one non-specific β-fructofuranosidase were found in a cell-free extract. The molecular weight of the endolevanases were estimated to be 60 and 36 kDa, whereas that of β-fructofuranosidase, 87 kDa. The former of the specific enzymes was associated with the outer membrane, while the latter and the non-specific β-fructofuranosidase, with the periplasm or cytosol. The Km and Vmax for Timothy grass fructan degradation by endolevanase were 0.27% and 15.75 μM fructose equivalents × mg protein?1 × min?1, those for sucrose and inulin digestion by β-fructofuranosidase were 1.35 × 10?3 M and 1.73 μM hexoses × mg protein?1 × min?1 and 1.77% and 1.83 μM hexoses × mg protein?1 × min?1, respectively. |
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