Prostaglandin E1 (PGE1), but not prostaglandin E2 (PGE2), alters luteal and endometrial luteinizing hormone (LH) occupied and unoccupied LH receptors and mRNA for LH receptors in ovine luteal tissue to prevent luteolysis |
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| Authors: | YS Weems TM Nett LA Rispoli TL Davis DL Johnson T Uchima A Raney E Lennon J Pang T Harbert G Bowers K Goto A Ong N Tsutahara RD Randel CW Weems |
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| Institution: | 1. Dept. of HNFAS, University of Hawaii, 1955 East-West Road, Honolulu, HI 96822, United States;2. College of Veterinary Medicine and Biomedical Sciences, Colorado State University, United States;3. Texas AgriLife Research, Overton, United States;1. Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand;2. Department of Animal Sciences, North Dakota State University, Fargo, ND, USA;3. Agricultural Biotechnology Research Center for Sustainable Economy (ABRCSE), Khon Kaen University, Khon Kaen, Thailand;1. Department of Comparative Biomedical Sciences, The Royal Veterinary College, Hertfordshire, University of London, UK;2. Department of Veterinary Medicine, Section of Obstetrics and Gynaecology, University of Sassari, Sassari, Italy;3. L''Institut National de la Recherche Agronomique, Unité Mixte de Recherche 6175, Physiologie de la Reproduction et des Comportements, Nouzilly, France;1. Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, University of Mehmet Akif Ersoy, Burdur, Turkey;2. Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, University of Dicle, Diyarbakir, Turkey;3. Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, University of Yuzuncu Yil, Van, Turkey;4. Clinic for Obstetrics, Gynecology and Andrology, Justus-Liebig-University, Giessen, Germany;1. Department of Human Nutrition, Food, and Animal Sciences, University of Hawaii, Honolulu, Hawaii, USA;2. College of Veterinary and Biomedical Sciences, Colorado State University, Fort Collins, USA;3. Texas AgriLife Research, Texas A&M System, Overton, USA;1. Laboratory of Mammalian Reproductive Biology and Genomics, Department of Animal Science, Michigan State University, East Lansing, MI 48824, USA;2. Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel;1. Eutheria Foundation, Cross Plains, Wisconsin, USA;2. Departamento de Medicina Veterinária, Escola de Ciências e Tecnologia, Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Instituto de Investigação e Formação Avançada, Universidade de Évora, Évora, Portugal;3. Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin–Madison, Madison, Wisconsin, USA |
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| Abstract: | Loss of luteal progesterone secretion at the end of the ovine estrous cycle is via uterine PGF2α secretion. However, uterine PGF2α secretion is not decreased during early pregnancy in ewes. Instead, the embryo imparts a resistance to PGF2α. Prostaglandins E (PGE; PGE1 + PGE2) are increased in endometrium and uterine venous blood during early pregnancy in ewes to prevent luteolysis. Chronic intrauterine infusion of PGE1 or PGE2 prevents spontaneous or IUD, estradiol-17β, or PGF2α-induced premature luteolysis in nonbred ewes. The objective was to determine whether chronic intrauterine infusion of PGE1 or PGE2 affected mRNA for LH receptors, occupied and unoccupied receptors for LH in luteal and caruncular endometrium, and luteal function. Ewes received Vehicle, PGE1, or PGE2 every 4 h from days 10 to 16 of the estrous cycle via a cathether installed in the uterine lumen ipsilateral to the luteal-containing ovary.Jugular venous blood was collected daily for analysis of progesterone and uterine venous blood was collected on day-16 for analysis of PGF2α and PGE. Corpora lutea and caruncular endometrium were collected from day-10 preluteolytic control ewes and day-16 ewes treated with Vehicle, PGE1 or PGE2 for analysis of the mRNA for LH receptors and occupied and unoccupied receptors for LH. Luteal weights on day-16 in ewes treated with PGE1 or PGE2 and day-10 control ewes were similar (P ≥ 0.05), but were greater (P ≤ 0.05) than in day-16 Vehicle-treated ewes. Progesterone profiles on days 10–16 differed (P ≤ 0.05) among treatment groups: PGE1 > PGE2 > Vehicle-treated ewes. Concentrations of PGF2α and PGE in uterine venous plasma on day-16 were similar (P ≥ 0.05) in the three treatment groups. Luteal mRNA for LH receptors and unoccupied and occupied LH receptors were similar (P ≥ 0.05) in day-10 control ewes and day-16 ewes treated with PGE2 and were lower (P ≤ 0.05) in day-16 Vehicle-treated ewes. PGE2 prevented loss (P ≤ 0.05) of day-16 luteal mRNA for LH receptors and occupied and unoccupied LH receptors. Luteal and caruncular tissue mRNA for LH receptors and occupied and unoccupied LH receptors were greater (P ≤ 0.05) on day-16 of PGE1-treated ewes than any treatment group. mRNA for LH receptors and occupied and unoccupied receptors for LH in caruncules were greater (P ≤ 0.05) in day-16 Vehicle or PGE2-treated ewes than in day-10 control ewes. It is concluded that PGE1 and PGE2 share some common mechanisms to prevent luteolysis; however, only PGE1 increased luteal and endometrial mRNA for LH receptors and occupied and unoccupied LH receptors. PGE2 prevents a decrease in luteal mRNA for LH receptors and occupied and unoccupied receptors for LH without altering endometrial mRNA for LH receptors or occupied and unoccupied receptors for LH. |
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