Sex in Penicillium: Combined phylogenetic and experimental approaches |
| |
| Authors: | M. López-Villavicencio G. Aguileta T. Giraud D.M. de Vienne S. Lacoste A. Couloux J. Dupont |
| |
| Affiliation: | 1. Origine, Structure, Evolution de la Diversité, UMR 7205 CNRS-MNHN, Muséum national d’histoire naturelle, CP39, 57 rue Cuvier, 75231 Paris Cedex 05, France;2. Ecologie, Systématique et Evolution, UMR 8079, Bâtiment 360, Université Paris-Sud, F-91405 Orsay cedex, France; UMR 8079, Bâtiment 360, CNRS, F-91405 Orsay cedex; France;3. Genoscope – Centre National de Séquençage: BP 191, 91006 EVRY cedex, France;1. Eternal University, Sirmour, Himachal Pradesh, India;2. Birla Institute of Technology, Ranchi, Jharkhand, India;3. ERA Chair of Green Chemistry, Tallinn University of Technology, Tallinn, Estonia;4. ICAR-National Bureau of Agriculturally Important Microorganisms, Kusmaur, Uttar Pradesh, India;1. Department of Genetics, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa;2. Crop Protection Division, Vegetable and Ornamental Plant Institute, Agricultural Research Council, Private Bag X293, Pretoria 0001, South Africa;1. UCD School of Agriculture and Food Science, University College Dublin, Dublin 4, Ireland;2. School of Environmental and Life Sciences, The University of Newcastle, PO Box 127, Ourimbah, NSW, 2258, Australia;3. Laboratory of Plant Pathology, Institute of Olive Tree Subtropical Crops and Viticulture, DEMETER, Chania, 73134, Crete, Greece;4. Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), CONICET-Facultad de Ciencias Exactas, Universidad Nacional de La Plata (UNLP), calle 47 y 116 (1900), La Plata, Argentina;5. Laboratory of Citrus Crops, Institute of Olive Tree Subtropical Crops and Viticulture, DEMETER, Chania, 73134, Crete, Greece |
| |
| Abstract: | We studied the mode of reproduction and its evolution in the fungal subgenus Penicillium Biverticillium using phylogenetic and experimental approaches. We sequenced mating type (MAT) genes and nuclear DNA fragments in sexual and putatively asexual species. Examination of the concordance between individual trees supported the recognition of the morphological species. MAT genes were detected in two putatively asexual species and were found to evolve mostly under purifying selection, although high substitution rates were detected at some sites in some clades. The first steps of sexual reproduction could be induced under controlled conditions in one of the two species, although no mature cleistothecia were produced. Altogether, these findings suggest that the asexual Penicillium species may have lost sex only very recently and/or that the MAT genes are involved in other functions. An ancestral state reconstruction analysis indicated several events of putative sex loss in the genus. Alternatively, it is possible that the supposedly asexual Penicillium species may have retained a cryptic sexual stage. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
