Vitek 2 ANC card versus BBL Crystal Anaerobe and RapID ANA II for identification of clinical anaerobic bacteria |
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| Authors: | Laurent Blairon Mengi L. Maza Ingrid Wybo Denis Piérard Anne Dediste Olivier Vandenberg |
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| Affiliation: | 1. Department of Microbiology, St-Pierre University Hospital, B-1000 Brussels, Belgium;2. Jules Bordet Institute, Brussels, Belgium;3. Department of Microbiology UZ Brussel University Hospital, Brussels, Belgium;1. Institute of Virology, National Reference Centre for Hepatitis C, Essen University Hospital, University of Duisburg-Essen, Hufelandstr., D-45122 Essen, Germany;2. Institute of Clinical and Molecular Virology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Schlossgarten 4, D-91054 Erlangen, Germany;3. Department of Gastroenterology and Hepatology, Essen University Hospital, University of Duisburg-Essen, Hufelandstr., D-45122 Essen, Germany;1. Institut hospitalo-universitaire (IHU) Méditerranée Infection, pôle des maladies infectieuses et tropicales clinique et biologique, fédération de bactériologie-hygiène-virologie, Assistance publique–Hôpitaux de Marseille, centre hospitalo-universitaire Timone, 264, rue Saint-Pierre, 13385 Marseille cedex 05, France;2. Aix-Marseille Université, URMITE UM63 CNRS 7278 IRD 198 Inserm U1095, facultés de médecine et de pharmacie, 27, boulevard Jean-Moulin, 13385 Marseille cedex 05, France;3. Service d’hépatogastroentérologie, centre hospitalo-universitaire Conception, 147, boulevard Baille, 13385 Marseille cedex 05, France;4. Service de pathologie digestive, hôpital d’instruction des armées Laveran, BP 60149, 13384 Marseille cedex 13, France;5. Hôpital de jour, centre hospitalier de Hyères, 8, rue Maréchal-Juin, Hyères, France;6. Service d’hépatogastroentérologie, centre hospitalo-universitaire Nord, chemin des Bourrelys, 13915 Marseille cedex 20, France;7. Service de transplantation hépatique, centre hospitalo-universitaire Conception, 147, boulevard Baille, 13385 Marseille cedex 05, France;8. Service d’anatomopathologie, centre hospitalo-universitaire Nord, chemin des Bourrely, 13015 Marseille, France;9. Service de réanimation, centre hospitalo-universitaire Conception, 147, boulevard Baille, 13385 Marseille cedex 05, France;1. Southern California Coastal Water Research Project, 3535 Harbor Blvd Ste 110, Costa Mesa, CA 92626, United States;2. UCLA Civil and Environmental Engineering, 5732H Boelter Hall, Los Angeles, CA 90095, United States;3. UCD School of Biomolecular and Biomedical Science, University College Dublin, Dublin 4, Ireland;4. Instituto Nacional de Saúde, Dr. Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisboa, Portugal;5. U.S. Geological Survey, Great Lakes Science Center, Lake Michigan Ecological Research Station, 1100 N. Mineral Springs Road, Porter, IN 46304, United States;6. UNC Chapel Hill Institute of Marine Sciences, 3431 Arendell Street, Morehead City, NC 28557, United States;7. Oak Ridge Institute for Science and Education, PO Box 117, Oak Ridge, TN 37831, United States;8. Environmental Microbiology and Molecular Ecology Group, Interuniversity Cooperation Centre for Water and Health, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorfer Strasse 1a/166-5-2, A-1060 Vienna, Austria;9. Department of Environmental Sciences and Engineering, University of North Carolina, 135 Dauer Drive, Chapel Hill, NC 27599-7431, United States;10. The Cooperative Institute for Marine and Atmospheric Studies, University of Miami, Miami, FL 33149, United States;11. Laboratoire de Microbiologie, MIC/LNR, Département Ressources Biologiques et Environnement, Unité Environnement, Microbiologie et Phycotoxines, Ifremer, ZI Pointe du diable, Plouzané, France;12. College of Public Health, Division of Environmental Health Science, The Ohio State University, 406 Cunz Hall, 1841 Neil Avenue, Columbus, OH 43210, United States;13. Source Molecular Corporation, 4985 SW 74th Court, Miami, FL 33155, United States;14. U.S. Environmental Protection Agency, Office of Research and Development, National Risk Management Research Laboratory, 26 West Martin Luther King Drive, Cincinnati, OH 45268, United States;15. Department of Fisheries and Wildlife, 13, Natural Resources, Michigan State University, East Lansing, MI 48824, United States;p. Department of Civil & Environmental Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, United States;q. National Oceanic and Atmospheric Administration, Atlantic Oceanographic and Meteorological Laboratory, 4301 Rickenbacker Causeway, Miami, FL 33149, United States;r. Earth Research Institute and Bren School of Environmental Science & Management, University of California, Santa Barbara, CA 93106, United States;s. Stanford University, Dept. of Civil & Environmental Engineering, 473 Via Ortega, Stanford, CA 94305, United States;t. Singapore Centre on Environmental Life Sciences Engineering (SCELSE), School of Biological Sciences SBS-B1N-27 and School of Civil and Environmental Engineering, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore;1. Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, Eötvös utca 6., 6720, Szeged, Hungary;2. Institute of Clinical Microbiology, Faculty of Medicine, University of Szeged, 6725, Szeged, Semmelweis utca 6., Hungary |
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| Abstract: | The Vitek 2 Anaerobe and Corynebacterium Identification Card (ANC) was recently evaluated in a multicentre study. In the present work, this system was compared with the BBL Crystal Anaerobe and RapID ANA II panels. These kits were tested using 196 strains of anaerobes that had been previously identified by gas–liquid chromatography. Identification to the species or to the genus level was 75.0%, 81.1% and 70.9% for Crystal, RapID and Vitek, respectively. Vitek ANC failed to provide any identification in 20.4% of the strains, but it had fewer misidentifications than RapID. The confidence factors provided on the results report of each kit were not always correlated with a lower risk of major errors, with the exception of Vitek 2 in which a confidence factor higher than 0.86 excluded the risk of misidentification in more than 87% of isolates. The lower rate of identification by the Vitek and Crystal panels is mostly due the lower ability of these systems to identify the Clostridia. Overall, the three panels are comparable but need improvement to a better accuracy. |
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