Phytophthora nicotianae transformants lacking dynein light chain 1 produce non-flagellate zoospores |
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| Authors: | Reena D. Narayan Leila M. Blackman Weixing Shan Adrienne R. Hardham |
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| Affiliation: | 1. Department of Plant and Animal Sciences, Faculty of Agriculture, Dalhousie University, Truro NS B2N 5E3, Canada;2. Department of Biology, Dalhousie University, Halifax NS B3H 4R2, Canada;3. Agriculture and Agri-Food Canada, Charlottetown PE C1A 4N6, Canada;4. Cavendish Farms, Kensington PE C0B 1M0, Canada;5. New Brunswick Department of Agriculture, Aquaculture and Fisheries, Wicklow NB E7L 3S4, Canada;6. National Research Council Institute for Marine Biosciences, Halifax NS B3H 3Z1, Canada;1. INRA, UMR1065 Santé et Agroécologie du Vignoble, Villenave d''Ornon, France;2. Université de Bordeaux, ISVV, UMR1065 SAVE, Villenave d''Ornon, France;1. Department of Forest Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Box 7026, SE-75007 Uppsala, Sweden;2. Department of Plant Protection Biology, Swedish University of Agricultural Sciences, Box 102, SE-23053 Alnarp, Sweden;3. BGI-Shenzhen, Main Building 11/F, Beishan Industrial Zone, Yantian District, Shenzhen 518083, China;4. Bioinformatics Infrastructure for Life Sciences, Department of Immunotechnology, Lund University, Medicon Village, 22381 Lund, Sweden |
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| Abstract: | Biflagellate zoospores of the highly destructive plant pathogens in the genus Phytophthora are responsible for the initiation of infection of host plants. Zoospore motility is a critical component of the infection process because it allows zoospores to actively target suitable infection sites on potential hosts. Flagellar assembly and function in eukaryotes depends on a number of dynein-based molecular motors that facilitate retrograde intraflagellar transport and sliding of adjacent microtubule doublets in the flagellar axonemes. Dynein light chain 1 (DLC1) is one of a number of proteins in the dynein outer arm multiprotein complex. It is a 22 kDa leucine-rich repeat protein that binds to the catalytic motor domain of the dynein γ heavy chain. We report the cloning and characterization of DLC1 homologues in Phytophthora cinnamomi and Phytophthora nicotianae (PcDLC1 and PnDLC1). PcDLC1 and PnDLC1 are single copy genes that are more highly expressed in sporulating hyphae than in vegetative hyphae, zoospores or germinated cysts. Polyclonal antibodies raised against PnDLC1 locallized PnDLC1 along the length of the flagella of P. nicotianae zoospores. RNAi-mediated silencing of PnDLC1 expression yielded transformants that released non-flagellate, non-motile zoospores from their sporangia. Our observations indicate that zoospore motility is not required for zoospore release from P. nicotianae sporangia or for breakage of the evanescent vesicle into which zoospores are initially discharged. |
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